Occurrence and molecular identification of an invasive rice strain of fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) from Sindh, Pakistan, using mitochondrial cytochrome c oxidase I gene sequences
نویسندگان
چکیده
Abstract The fall armyworm (FAW), an invasive pest of maize, is emerging threat in Southern Asia after America and Europe. Recently, this notorious has also been found different areas Pakistan. To assess its presence Pakistan, a survey was carried out the provinces Punjab, Sindh, Khyber Pakhtunkhwa during May–October 2019. We observed highest incidence FAW Sindh with maximum impact districts Tando-Allahyar Hyderabad. These samples were identified as Spodoptera frugiperda on morphological taxonomical bases. However, identification very difficult at early larval instars. Here, we use mitochondrial cytochrome c oxidase I (COI) gene region for precise larva species level. Two regions COI (COI-5′ COI-3′) used molecular markers species. DNA sequence similarity searches obtained sequences (NCBI GenBank Accession Nos. MW241537, MW241538, MW349515, MW349516, MW349493 MW349494) revealed that genetically it more than 99% identical to S. . phylogenetic analysis indicated rice-strain (R-strain). Both 3′- 5′-fragment tree topologies showed collected belong R-strain. best our knowledge, first report providing evidence existence R-strain feeding maize crop using marker.
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ژورنال
عنوان ژورنال: Journal of Plant Diseases and Protection
سال: 2021
ISSN: ['1861-3837', '1861-3829']
DOI: https://doi.org/10.1007/s41348-021-00548-6